Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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Replacement fluorescence emission filter supplied with a mounting holder for use in compatible Molecular Devices microplate readers. The filter provides a red-region emission band centered at 625 nm with a 35 nm full bandwidth, suitable for fluorescence intensity and time-resolved fluorescence assays.
- Center wavelength 625 nm.
- Bandwidth 35 nm full width.
- Includes mounting holder for slide insertion.
- Optimized for red-emission fluorescence detection.
- Intended for use in microplate readers that accept plug-in filters.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Fluorescence interference filter supplied with a mounting holder for insertion into compatible filter-slide microplate readers. Intended for near-infrared fluorescence detection in microplate assays.
- Center wavelength 740 nm.
- Bandwidth 50 nm.
- Includes mounting holder for filter slides.
- Designed for fluorescence detection; suitable for luminescence and TRF read modes.
- Intended for microplate and ELISA assays using filter-based readers.
- Interference filter construction for stable optical performance.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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